Studies on the determination of diacetyl guanfubase a concentration in rabbit plasma and pharmacokinetics

✍ Scribed by Wei-hong Wang; Ru-bin Wang; Jun-ren Zhang; Chuan-hua Liu; Shu-ju Pan

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 39 KB
• Volume: 13
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199902)13:1<83::aid-bmc805>3.0.co;2-i

✦ Synopsis

A gas chromatographic method for monitoring diacetyl guanfubase A in plasma is described. The procedure involved a single solvent extraction of drug from rabbit plasma into ethyl acetate with guanfubase A as an internal standard. The extract was analyzed subsequently on a gas chromatograph equipped with a hydrogen flame ionization detector. The recovery was 86.43% AE 6.90% (AESD); the RSD of within-day and between-day was 2.81%-5.26% and 5.22%-8.24%, respectively; the regression line was linear over the concentration range of 25-200 mg/mL, the limit of detection was 10 mg/mL. No endogeneous interference was found in chromatograms of the biological samples. This method was applied to the pharmacokinetic study of diacetyl guanfubase A in rabbits.