Studies on synthetic pathway of xylose-containing N-linked oligosaccharides deduced from substrate specificities of the processing enzymes in sycamore cells (Acer pseudoplatanus L.)

Purification and Substrate-Specificity o

Purification and Substrate-Specificity of β-Xylosidase from Sycamore Cell (Acer-Pseudoplatanus L.): Application for Structural Analysis of Xylose-Containing N-Linked Oligosaccharides

✍ K. Tezuka; M. Hayashi; H. Ishihara; M. Nishimura; K. Onozaki; N. Takahashi 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 340 KB

A beta-xylosidase was purified 51-fold from culture medium of sycamore (Acer pseudoplatanus L.) cells using p-nitrophenyl beta-D-xylopyranoside as a substrate. This enzyme can remove a xylose residue from asparagine-linked oligosaccharides, derivatized with 2-aminopyridine. A pentasaccharide, Xy1 be