Studies on production of biologically active substance which inhibits the intracellular multiplication ofToxoplasmawithin mouse macrophages
✍ Scribed by Toshikazu Shirahata; Kiheiji Shimizu; Shigeru Noda; Naoyoshi Suzuki
- Book ID
- 104748499
- Publisher
- Springer-Verlag
- Year
- 1977
- Tongue
- English
- Weight
- 1022 KB
- Volume
- 53
- Category
- Article
- ISSN
- 1432-1955
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✦ Synopsis
In vitro assessments were carried out to study some biological aspects of immune lymphocytes producing a new lymphokine, called by the authors Toxoplasma growth inhibitory factor (Toxo-GIF), which inhibits the intracellular multiplication of Toxoplasma gondii within nonimmune mouse macrophages.
Concanavalin A and phytohemagglutinin-P were found to induce vigorous production of Toxo-GIF, whereas bacterial lipopolysaccharide did not. In vitro treatment of splenic lymphocytes with rabbit anti-mouse thymocyte serum plus complement abolished almost completely their ability to produce Toxo-GIF. Treatment of splenic lymphocytes with inhibitors of protein synthesis, cycloheximide or puromycin resulted in a remarkable reduction of the ability of sensitized lymphocytes to produce this lymphokine. Thus the production of Toxo-GIF seems to be dependent on the cellular metabolic events of sensitized Tlymphocytes.
The significant activity of Toxo-GIF was demonstrable even in the supernate of lymphocyte cultures incubated in serum-free medium and was also evident after immune lymphocytes and homologous antigen were incubated for the relatively short period of 10 h.