✦ LIBER ✦

Studies on polypeptides XXXIII: Semisynthetic Ribonuclease analogues. The role of histidine-119

✍ Scribed by J. Serdijn; C. Hoes; J. Raap; K. E. T. Kerling

Book ID: 104588271
Publisher: Elsevier Science
Year: 2010
Tongue: English
Weight: 446 KB
Volume: 99
Category: Article
ISSN: 0165-0513
DOI: 10.1002/recl.19800991107

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

As part of a structure‐activity relationship study of ribonuclease, focussing on the role of histidine‐119 in the active centre, two tetradecapeptides were synthesized by the solid‐phase method: [His^119^]RNase 111‐124 and the corresponding analogue in which His‐119 is replaced by__L__ homohistidine.

While the [Hhis^119^] analogue has almost the same binding capacity towards RNase 1‐118 protein as the natural peptide, the resulting complex is devoid of any catalytic activity when RNA or 2′,3′‐CMP is used as substrate.

📜 SIMILAR VOLUMES

Studies on polypeptides XL. The role of

Studies on polypeptides XL. The role of histidine-119 in non-covalent semisynthetic ribonuclease; its replacement by 3-(3-pyrazolyl)-L-alanine, Nπ-methyl-L-histidine and Nτ-methyl-L-histidine

✍ J. Serdijn; W. Bloemhoff; K. E. T. Kerling; E. Havinga 📂 Article 📅 2010 🏛 Elsevier Science 🌐 English ⚖ 536 KB

Studies on polypeptides XLI1H NMR studie

Studies on polypeptides XLI1H NMR studies of non-covalent, semi-synthetic ribonuclease A analogues in which histidine-119 has been replaced by L-homohistidine, Nτ-methyl-L-histidine, Nπ-methyl-L-histidine and 3-(3-pyrazolyl)-L-alanine, respectively

✍ J. Serdijn; W. Bloemhoff; K. E. T. Kerling; E. Havinga 📂 Article 📅 2010 🏛 Elsevier Science 🌐 English ⚖ 866 KB

1H-NMR study on the tautomerism of the i

1H-NMR study on the tautomerism of the imidazole ring of histidine residues: II. Microenvironments of histidine-12 and histidine-119 of bovine pancreatic ribonuclease a

✍ Masaru Tanokura 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 725 KB

Studies on polypeptides XXXI. An approac

Studies on polypeptides XXXI. An approach to the chemical semisynthesis of ribonuclease A analogues. Part I: The preparation of N-acetimidoyl-S-protein

✍ P. Hoogerhout; W. Bloemhoff; K. E. T. Kerling 📂 Article 📅 2010 🏛 Elsevier Science 🌐 English ⚖ 531 KB

Studies on polypeptides XXXIX. The role

Studies on polypeptides XXXIX. The role of the imidazole tele-nitrogen atom of histidine-12 in the catalytic action of RNase S′

✍ C. Hoes; K. E. T. Kerling; E. Havinga 📂 Article 📅 2010 🏛 Elsevier Science 🌐 English ⚖ 911 KB

Studies on polypeptides XXXVIII. An appr

Studies on polypeptides XXXVIII. An approach to the chemical semisynthesis of ribonuclease a analogues. Part II. Semisynthesis of alanyl-Nϵ-octaacetimidoyl-S-protein and Nϵ-octaacetimidoyl-ribonuclease(14–124)

✍ P. Hoogerhout; K. E. T. Kerling 📂 Article 📅 2010 🏛 Elsevier Science 🌐 English ⚖ 595 KB