Studies on molecular weights of polysaccharides of Auricularia auricula-judae

✍ Scribed by Lina Zhang; Liqun Yang; Qiong Ding; Xianfeng Chen

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 448 KB
• Volume: 270
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(94)00008-4

✦ Synopsis

Three D-glucans (A, C, and E) and two acidic heteropolysaccharides (D and B), isolated from the fruit bodies of Auricularia auricula-judae (which grows in Fangshan, Hubei, China) were analysed by paper chromatography, gas chromatography, and IR and 13C NMR. Glucans A, C, and E consist mainly of a backbone chain of/3-(1 ~ 3)-D-glucose residues, with various branched groups. Polysaccharides D and B contain residues of D-xylose, o-mannose, D-galactose, D-glucose, and D-glucuronic acid. The weight-average molecular weights Mw, number-average molecular weights M,, intrinsic viscosities [~], and Huggins constants k' of these polysaccharides were studied by light scattering, membrane osmometry, and viscometry. The values of Mw for samples of A, C, E, D, and B are 117 × 104, 144 × 104, 200 × 104, 30 × 104, and 50 x 104, respectively. Analysis of the Mw, M,, [~7], and k' data indicates polysaccharides A and E to have stiff chains, and C, D, and B flexible chains in aqueous solutions.