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Studies on intercellular adhesion. III. Adhesion of cells to isotypic or heterotypic collecting particles and monolayers

✍ Scribed by Bernard Pessac; Arlette Girard; Françoise Alliot

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
509 KB
Volume
98
Category
Article
ISSN
0021-9541

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✦ Synopsis

Adhesion between cells of different tissues from chick embryos was studied by the modified collecting particle (Pessac et al., '77a) and stationary monolayer assays. These assays measure the number of 3H labeled cells that adhere to the surface of cell aggregates and tissue fragments or on top of cell monolayers. The numbers of cells from a given suspension that adhere to identical or different cells can be compared. Intercellular adhesion may be considered as tissue specific if, in identical conditions, more cells adhere to identical cells than to cells from different tissues. Neural retina, cerebrum, optic tectum, liver and kidney cells from 9-day-old chick embryos showed no tissue specificity of adhesion, while heart cells adhered preferentially to collecting heart fragments.

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Studies on intercellular adhesion. I. Ad
Studies on intercellular adhesion. I. Adhesion of neural retina cells to isotypic aggregates in serum free medium
✍ Bernard Pessac; Françoise Alliot; Arlette Girard 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 586 KB

## Abstract A quantitative assay for intercellular adhesion, which is a modification of the collecting aggregate assay (Roth et al., 1971) is described. The use of mechanically dissociated single cells labeled with ^3^H‐leucine, and of a gyratory shaker increased considerably the efficiency of cell

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Studies on intercellular adhesion. II. Promoting effect of serum and proteins on adhesion of neural retina cells to isotypic aggregates
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## Abstract The quantitative assay described in the preceding paper (Pessac et al., 1977) was used to study the effects of serum and various proteins on isotypic adhesion of chick embryo neural retina cells. Fetal bovine, chicken, horse, rabbit and human sera promoted cell adhesion to the same exte