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Studies on glutamic-oxalacetic (GOT) and glutamic-pyruvic (GPT) transaminases of swine kidney wormStephanurus dentatus(Diesing, 1839)

✍ Scribed by R. K. Sharma; Kavindra Singh

Publisher: Springer-Verlag
Year: 1977
Tongue: English
Weight: 303 KB
Volume: 54
Category: Article
ISSN: 1432-1955
DOI: 10.1007/bf00390116

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✦ Synopsis

Biochemical studies on the two transaminases GOT and GPT of swine kidney worm Stephanurus dentatus have been made. GOT has been found much more active than GPT. Enzyme activities are based on the formation of oxaloacetate (GOT) or pyruvate (GPT) from aspartic acid and alanine respectively with oxoglutarate. A linear relationship is observed between the enzyme concentration and activity. GOT shows a maximum activity at pH 8.0 and Michaelis constant 9 X 10(-3) M for male and 2.9 X 10(-3) M for female. GPT has an optimum pH of 7.5 and a Michaelis constant 19 X 10(-3) M for male and 8 X 10(-3) M for female. The optimum temperature for both GOT and GPT was 60 degrees C.

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