A highly sens,tive fluorescent staining method for detecting glycosylated proteins on SDS-polyacrylamide gel has been described. The carbohydrate groups in glycoproteins were oxidized with periodate on SDS-polyacrylamide gel to generate aldehyde groups which were subsequently labeled with a fluoresc
Studies of the influence of the surfactant sodium dodecyl sulfate on the fluorescence properties of kinetin
β Scribed by Hui Wu; Huaigong Wang
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 268 KB
- Volume
- 329
- Category
- Article
- ISSN
- 0003-2670
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β¦ Synopsis
A detailed study of the influence of an anionic surfactant (sodium dodecyl sulfate, SDS) on the fluorescence properties of kinetin with NaHSO, at pH 1.4-2.4 (X(ex) = 325 nm, X(em) = 500 nm) has been undertaken. In the presence of SDS, the fluorescence intensity is 9.5 times greater that in its absence. The detection limit for kinetin is 0.21 ng ml-', and the calibration graph is linear over the range of 0.214000 ng ml-'.
π SIMILAR VOLUMES
The effect of medium chain length alcohols on the micellar size and shape of sodium dodecyl sulfate in electrolyte solutions has been investigated by means of small angle neutron scattering (SANS), dynamic light scattering (DLS), and viscosity measurements. The surfactant content was kept constant t