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Studies of the influence of nicotinamide on the development of heat resistance in Bacillus cereus T

✍ Scribed by D. K. Kalita; Dr. R. P. Singh

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
332 KB
Volume
27
Category
Article
ISSN
0233-111X

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✦ Synopsis

Effects of nicotinamide (NA) on the development of heat resistance and sporulation in Bacillus cereus T were studied. It was observed that NA produces more than 95% heat labile spores (80 "C for 30 min). The growth and the utilization of poly-B-hydroxybutyrate (PHB) is not affected. The heat labile spores were morphologically comparable to untreated spores but the levels of spore specific proteins and dipicolinic acid (DPA) were only 1 5 -2 0 ~0 of the maximum. NA-treated spores germinate poorly ( w 10%) and a storage of 2 months a t 4 "C causes complete loss of germination characteristics. However, the exogenous addition of DPA or L-kynurenine completely reverses all effects indicating a possible involvement of L-tryptophan metabolism in the development of heat resistance.

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