Studies of polypeptide structure by fluorescence techniques. III. Interaction between dye and macromolecule in fluorescent conjugates

✍ Scribed by Thomas J. Gill III; E. M. McLaughlin; Gilbert S. Omenn

• Publisher: Wiley (John Wiley & Sons)
• Year: 1967
• Tongue: English
• Weight: 675 KB
• Volume: 5
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1967.360050307

✦ Synopsis

Synopsis

'The effects of p11 on the polarization of flnoresrence of dyes dissolved in media of high visrosit y or conjugated to polypeptides that undergo no structural transitions indicate that DNS is useful for studying pH-dependent molecular transition over the range p H 2.5-14, whereas fluorescein is useful only over the range pH 6-8. Heating and cooling in aqueous solutions cause no change in the polarization of flnorescein or of DNS; there-* The nomenclature of the synthetic polypeptides was systematically defined previoiisly.~6 The snpersc4plh are lhe moliw pewelitage of each amino miid residue anti the nunil)er f o l l ~i ~i g f lie polypeptide fornmla tleiiotes the preparnt ioti. Other atitirevi:t-1 ions are PVAm, polyvin3.l:tmiiie; Fit(*, fliioresceiti isolhioc.3.a11:~te; I)NH, l-diniet hylaminonaphl hnleiie-5-sulfoityl chloride; 1)NS. S() ,TI, 1 -dimel 1~ylsm1iioriaphthaleiie-T,siilfonic acid; atid Iisec., natiosecoiid (lOP see.).