Studies of Iron-Sulfur Covalency in the Model System and Proteins
โ Scribed by Xiu-Juan Qin; Yan Chen; Fa-Ming Gao
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 94 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0256-7660
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โฆ Synopsis
Abstract
It was found that the highly covalent nature of the metalโligand interactions in the FeโS cluster clearly played an important role in determining the reactivity of the sites. A semiโempirical model, based on the Phillips theory of bonding was developed for quantitative explanation of covalency in FeโS cluster, showing that Mossbauer spectroscopy and electronic absorption spectroscopy provided the direct experimental probe of covalency of FeโS~4~ clusters.
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## Abstract The model ironโsulfur (FeโS) protein ferredoxin (Fd) from __Synechocystis sp__. PCC 6803 has been simultaneously produced and matured in a cellโfree production system. After 6 h of incubation at 37ยฐC, Fd accumulated to >450 ยตg/mL. Essentially all was soluble, and 85% was active. Product