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Studies of colicin induction with an imm- col+ mutant of the plasmid colicin E1

โœ Scribed by Inselburg, Joseph

Publisher
Springer
Year
1979
Tongue
English
Weight
625 KB
Volume
173
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

A mutant of a derivative of the colicin E1 plasmid has been isolated that does not confer immunity to colicin E1 on its host (imm-) although it is still capable of producing colicin (col+). Cells carrying the col+, imm- plasmid are capable of forming colonies and grow best in liquid culture in the presence of trypsin. The induction of colicin synthesis by ultraviolet light has been analysed using this mutant plasmid. The results suggest that a) the expression of the col+ gene may be delayed for many generations after the inducing stimulus, b) although induced cells are usually killed they can reproduce and c) the capacity to produce colicin can be propagated and segregated into the progeny of an induced cell.

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An Escherichia coli mutant thermosensitive in the B subunit of DNA gyrase: Effect on the structure and replication of the colicin E1 plasmid in vitro
โœ Orr, Elisha ;Staudenbauer, Walter L. ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 460 KB

An E. coli strain which carries a mutation conferring clorobiocin resistance and temperature sensitivity for growth has recently been described and evidence has been presented suggesting that the mutation is located in the gyrB gene (Orr et al. 1979). The replication of the ColE1 plasmid was analyse