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Studies of carcino-fetal proteins: Physical and chemical properties of human α-fetoprotein

✍ Scribed by Erkki Ruoslahti; Markku Seppälä

Publisher
John Wiley and Sons
Year
1971
Tongue
French
Weight
518 KB
Volume
7
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

To obtain information on the relationship between a carcino‐fetal protein, α‐fetoprotein, fetal proteins from other species and other human serum proteins including the fetoprotein in patients with hepatocellular cancer, α‐fetoprotein was purified and characterized from the serum of human fetuses. The protein is composed of a single polypeptide chain. A molecular weight of 70,000 was obtained by gel electrophoresis in the presence of SDS and by gel filtration. The isoelectric point of α‐fetoprotein was found to be 4.75. It contains 4.3% carbohydrate with 2 moles of sialic acid per mole of protein. The amino acid composition of α‐fetoprotein was determined.

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