Structures of UV-B Induced Sunscreen Pigments of the Scots Pine (Pinus sylvestris L.)

✍ Scribed by Dipl.-Chem. Tim P. Jungblut; Dr. Jörg-P. Schnitzler; Dr. Werner Heller; Prof. Dr. Heinrich Sandermann Jr.; Dr. Norbert Hertkorn; Dr. Wilfried Szymczak; Dr. Jörg W. Metzger

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 389 KB
• Volume: 34
• Category: Article
• ISSN: 0044-8249
• DOI: 10.1002/anie.199503121

✦ Synopsis

needles (1 from less than 0.1 to about 0.9 pmol per g fresh weight, and 2 from 0.8 to 2.4 pmol per g fresh weight).I6] Figure 1 shows a typical HPLC elution profile of a methanolic extract from cotyledons after four days of UV-B treatment.

Compounds 1 and 2 were purified and isolated by countercurrent chromatography (MLCCC), gel chromatography on Sephadex LH-20, and preparative RP-HPLC. Their structures were determined by UV and ' H N M R spectroscopy (2D 'H-lH COSY and NOESY), modern techniques in mass spectrometry (TOF-SIMS, electrospray MS in combination with MS/MS). as well as selective chemical degradation. According to these results, 1 is 3",6"-di-O-(pava-coumaroyl)isoquercitrin. and 2 is 3",6"-di-O-(para-coumaroyl)astragalin. These two metabolites