Structure of transfer RNA—Aminoacyl transfer RNA synthetase complexes investigated by nuclease digestion
✍ Scribed by Leon A. Dickson; Paul R. Schimmel
- Book ID
- 115702758
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 764 KB
- Volume
- 167
- Category
- Article
- ISSN
- 0003-9861
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📜 SIMILAR VOLUMES
The aminoacyl-tRNA synthetases are an ancient group of enzymes that catalyze the covalent attachment of an amino acid to its cognate transfer RNA. The question of specificity, that is, how each synthetase selects the correct individual or isoacceptor set of tRNAs for each amino acid, has been referr
A continuous spectrophotometric assay is described for the aminoacylation reaction catalyzed by Escherichia coli alanyl-transfer RNA synthetase. The assay is based on coupling the alanyl-tRNA synthetase-dependent formation of AMP to the lactate dehydrogenase oxidation of NADH. Oxidation of NADH, as