Structure of the sugar-phosphate moiety of lipid A from lipooligosaccharide ofNeisseria meningitidisgroup B, strain BC5S No. 125
✍ Scribed by Vyacheslav L. L'vov; Irina K. Verner; Larisa Yu. Musina; Alexander V. Rodionov; Anatoly V. Ignatenko; Alexander S. Shashkov
- Book ID
- 104680364
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 484 KB
- Volume
- 157
- Category
- Article
- ISSN
- 0302-8933
No coin nor oath required. For personal study only.
✦ Synopsis
On the basis of chemical and NMR data the partial structure of lipid A from lipooligosaccharide (LOS) of Neisseria meningitidis group B, strain BC5S No 125 was established. Lipid A consisted of disaccharide 2-deoxy-6-O-[2-deoxy-2-(3-hydroxytetradecanoylamino)- beta-gluco-pyranosyl]-2-(3-hydroxytetradecanoylamino)- alpha-glucopyranose carrying the beta-(2-aminoethyl)pyrophosphate residue at 0-4' and the pyrophosphate or phosphate residue at 0-1. On hydrolysis of the acidic form of LOS with 1% acetic acid the substituent at 0-1 was practically completely removed whereas that at 0-4' was stable. The analogous hydrolysis of the Mg-salt of LOS was accompanied by splitting off the pyrophosphate linkage in the substituent at 0-4'. Hydrolysis of LOS at pH 4.5 in the presence of SDS led mainly to a lipid A preparation retaining both pyrophosphate residues.