## Abstract The bldB gene from __Streptomyces lividans__ was cloned, and its product was overexpressed in __Escherichia coli__ using a T7 expression system. Gel mobility shift assays showed that the BldB protein was functionally expressed in the __E. coli__ system and may negatively regulate its ow
Structure of the dna binding cleft of the gene 5 protein from bacteriophage fd
โ Scribed by McPherson, Alexander ;Jurnak, Frances ;Wang, Andrew ;Rich, Alexander ;Molineux, Ian
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1979
- Tongue
- English
- Weight
- 559 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0091-7419
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This paper considers how enzymes that catalyze reactions at specific DNA sites have been engineered to overcome the problem of competitive inhibition by excess nonspecific binding sites on DNA. The formation of a specific protein-DNA recognition complex is discussed from both structural and thermody