Structure of the d-mannan of Candida stellatoidea IFO 1397 strain. Comparison with that of the phospho-d-mannan of Candida albicans NIH B-792 strain

✍ Scribed by Minehiro Tojo; Nobuyuki Shibata; Yohko Ban; Shigeo Suzuki

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 776 KB
• Volume: 199
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(90)84263-t

✦ Synopsis

The structure of the D-mannan of Candida stellatoidea IFO 1397 strain, which has properties identical to those of the phospho-D-mannan of C. albicans serotype B strain, does not contain phosphate groups, and its 1H- and 13C-n.m.r. spectra are quite similar to those of the phospho-D-mannan of C. albicans NIH B-792 strain. However, the 1H-n.m.r. and 1H-13C-correlation n.m.r. spectra of the products obtained by digestion with alpha-D-mannosidase of C. stellatoidea D-mannan considerably differed from those of the corresponding digestion products of the C. albicans phospho-D-mannan. Additionally, the enzyme-linked immunosorbent assay, by means of a monoclonal antibody corresponding to (1----2)-linked beta-D-oligomannosyl residues, of the phospho-D-mannan of the same C. albicans strain indicated that the C. stellatoidea D-mannan does not contain any (1----2)-linked beta-D-oligomannosyl residues. The absence of these residues may be used as one of the criteria of chemotaxonomical identification of C. stellatoidea spp.