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Structure of Spin-Labeled Methylmethanethiolsulfonate in Solution and Bound to TEM-1 β-Lactamase Determined by Electron Nuclear Double Resonance Spectroscopy †

✍ Scribed by Mustafi, Devkumar; Sosa-Peinado, Alejandro; Gupta, Vanita; Gordon, David J.; Makinen, Marvin W.


Book ID
127350669
Publisher
American Chemical Society
Year
2002
Tongue
English
Weight
851 KB
Volume
41
Category
Article
ISSN
0006-2960

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The conformation of the nitroxyl spin-label ethyi 3-(2,2,5,5-tetramethylpyrrolinyl-1-oxy1)-propen-2-oate has been determined by electron nuclear double resonance (ENDOR) spectroscopy and computer-based molecular modeling. From ENDOR spectra of the compound in frozen solution, we have assigned resona