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Structure of pressure-induced denatured state of human serum albumin: a comparison with the intermediate in urea-induced denaturation

✍ Scribed by Naoki Tanaka; Hitoshi Nishizawa; Shigeru Kunugi


Book ID
114149313
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
230 KB
Volume
1338
Category
Article
ISSN
0167-4838

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The unfolding of human serum albumin (HSA), a multidomain protein, by urea was followed by far-UV circular dichroism (CD), intrinsic fluorescence, and ANS fluorescence measurements. The urea-induced transition, which otherwise was a two-step process with a stable intermediate at around 4.8 M urea co