Structure of horse-spleen apoferritin at 6 Å resolution
✍ Scribed by HOARE, R. J.; HARRISON, P. M.; HOY, T. G.
- Book ID
- 109695694
- Publisher
- Nature Publishing Group
- Year
- 1975
- Tongue
- English
- Weight
- 235 KB
- Volume
- 255
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/255653a0
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📜 SIMILAR VOLUMES
Crystallographic studies of L-chain horse spleen apoferritin (HSF) co-crystallized with Pt-hematoporphyrin IX and Snprotoporphyrin IX have brought significant new insights into structure-function relationships in ferritins. Interactions of HSF with porphyrins are discussed. Structural results show t
Apoferritin from horse spleen can be reversibly dissociated at pH2 or in 7.2 M G-HCl (pH 3.5). Reconstitution of the native icositetramer in 0.1 M TEA buffer (pH 7.9) in the presence of 1 mM EDTA and 3 mM dithioerythritol leads to yields higher than 80%. To monitor the kinetic mechanism, intrinsic f