Structure of Escherichia coli ribonucleotide reductase R2 in space group P6122

✍ Scribed by Sommerhalter, Monika ;Saleh, Lana ;Bollinger, J. Martin ;Rosenzweig, Amy C.

• Book ID: 104478168
• Publisher: International Union of Crystallography
• Year: 2005
• Tongue: English
• Weight: 619 KB
• Volume: 61
• Category: Article
• ISSN: 0907-4449
• DOI: 10.1107/s0907444905034062

✦ Synopsis

A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6 1 22 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl 2 or MnCl 2 results in full occupancy of the dinuclear metal site. The structure of the Mn II -loaded form was determined to 2.6 A resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms.