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Structure of Escherichia coli membranes. Fatty acyl chain order parameters of inner and outer membranes and derived liposomes

✍ Scribed by Gally, Hans Ulrich; Pluschke, Gerd; Overath, Peter; Seelig, Joachim

Book ID
121411970
Publisher
American Chemical Society
Year
1980
Tongue
English
Weight
778 KB
Volume
19
Category
Article
ISSN
0006-2960

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A rapid and simple method for separating and isolating the inner and outer membranes of Escherichia coli is described. Membrane vesicles were prepared either by passing the bacteria through a French press or by conversion of the cells to spheroplasts by the lysozyme-EDTA treatment and disruption of