𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Structure of Bombyx mori chemosensory protein 1 in solution

✍ Scribed by Séverine Jansen; Josef Chmelík; Lukáš Žídek; Petr Padrta; Petr Novák; Zbyněk Zdráhal; Jean-François Picimbon; Christer Löfstedt; Vladimír Sklenář


Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
361 KB
Volume
66
Category
Article
ISSN
0739-4462

No coin nor oath required. For personal study only.

✦ Synopsis


Abstract

Chemosensory Proteins (CSPs) represent a family of conserved proteins found in insects that may be involved in chemosensory functions. BmorCSP1 is expressed mainly in antennae and legs of the silkworm moth Bombyx mori and was cloned from antennal cDNA. Here we report the determination of the structure of Bombyx mori CSP1 (BmorCSP1) by NMR. The overall fold of BmorCSP1 is globular and comprises six α‐helices. These helices span residues 10–14, 17–27, 35–49, 57–72, 75–85, and 92–100. The internal hydrophobic sides of the helices are formed mostly by leucine and isoleucine residues and, therefore, well suited to constitute a binding site for hydrophobic ligands. Arch. Insect Biochem. Physiol. 66:135–145, 2007. © 2007 Wiley‐Liss, Inc.


📜 SIMILAR VOLUMES


Purification and molecular cloning of ch
✍ Jean-François Picimbon; Karen Dietrich; Sergio Angeli; Andrea Scaloni; Jürgen Kr 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 158 KB 👁 2 views

Soluble low molecular weight acidic proteins are suspected to transport stimulus molecules to the sensory neurons within insect sensilla. From the antennae of Bombyx mori, we have purified and sequenced a protein (BmorCSP1) bearing sequence similarity to a class of soluble chemosensory proteins rece

Sperm/DNA interaction: DNA binding prote
✍ Y. Shamila; S. Mathavan 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 117 KB 👁 1 views

Plasmid DNA transferred into testes of silkworm larvae following in vivo gene transfer method was taken up by the sperm cells during spermatogenesis and the DNA was transferred to Go and subsequent progeny. RT-PCR analysis confirmed the internalization of the DNA taken up by the sperm. Specific clas

The dissolution and characterization of
✍ Anshu Bagga Mathur; Alan Tonelli; Thomas Rathke; Sam Hudson 📂 Article 📅 1997 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 310 KB 👁 2 views

Bombyx mori silk fibroin from the silkworm was found to be soluble in a calcium nitrate-methanol system. Fibroin dissolves in 75% w/v Ca(NO 3 ) 2 /MeOH solution at a temperature of 67ЊC. The viscometric behavior of the fibroin-salt solution was analyzed and the fibroin's secondary structures were de