Structure of a bacterial photosynthetic membrane: Integrity of reaction centers following proteolysis and detergent solubilization

Quantitative Affinity Chromatographic St

Quantitative Affinity Chromatographic Studies of Mitochondrial Cytochrome c Binding to Bacterial Photosynthetic Reaction Center, Reconstituted in Liposome Membranes and Immobilized by Detergent Dialysis and Avidin–Biotin Binding

✍ Qing Yang; Xue-Ying Liu; Masayuki Hara; Per Lundahl; Jun Miyake 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 101 KB

In order to study the affinity binding of c-type cytochromes to the photosynthetic reaction center (RC) by quantitative affinity chromatography (QAC), RC from Rhodobacter sphaeroides was reconstituted into liposomes composed of egg phosphatidylcholine (EPC) and 2 mol% of biotinyl phosphatidylethanol