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Structure elucidation and antibacterial activity of a new coumarinolignoid from Daphne gnidium L.

โœ Scribed by F. Cottiglia; L. Bonsignore; G. Loy; D. Garau; C. Floris; M. Casu


Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
90 KB
Volume
40
Category
Article
ISSN
0749-1581

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โœฆ Synopsis


Abstract

Phytochemical investigation of the dichloromethane extract from the leaves of Daphne gnidium L. afforded 2โ€(4โ€hydroxyโ€3,5โ€dimethoxyphenyl)โ€3โ€hydroxymethylโ€2,3โ€dihydroโ€1,4,5โ€trioxaphenanthrenโ€6โ€one, and its structure was determined by a combination of spectral methods (1D and 2D ^1^H, ^13^C NMR and HRMALDI and EI mass spectrometry). The new compound was found to possess moderate antibacterial activity against Staphylococcus aureus and Bacillus cereus. However, it showed no cytotoxic activity against KB cells. Copyright ยฉ 2002 John Wiley & Sons, Ltd.


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reflux for 20 h and allowed to cool, and the nearly quantitatively precipitated dicyclohexylurea was filtered off and washed with a small amount of dichloromethane. The combined organic phases were concentrated in vacuum and the residue was dissolved in ether. The ether solution was washed with NaHC