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Structure-based substrate screening for an enzyme

✍ Scribed by Tao Xu; Lujia Zhang; Xuedong Wang; Dongzhi Wei; Tianbi Li


Book ID
114998724
Publisher
BioMed Central
Year
2009
Tongue
English
Weight
791 KB
Volume
10
Category
Article
ISSN
1471-2105

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Liposomes were prepared from 1-palmitoyl-2oleoyl-sn-glycero-3-phosphocholine (POPC), which contained the water soluble proteinase ␣-chymotrypsin. This liposome entrapped enzyme showed selectivity for externally added substrates in that only small substrates (benzoyl-L-Tyr-p-nitroanilide or acetyl-L-