Structure and reactivity of tRNA

✍ Scribed by Friedrich Cramer; Volker A. Erdmann; Friedrich Von Der Haar; Eckhard Schlimme

• Book ID: 102879897
• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: English
• Weight: 952 KB
• Volume: 74
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040740416

✦ Synopsis

All tRNA sequences so far known can be folded into a cloverleaf structure. Physical data and chemical reactions allow us to draw conclusions on secondary (cloverleaf ) and tertiary structure. N-oxidation of adenosine to adenosine-1-N-oxide can be done with monoperphthalic acid in non-base-paired regions of polynucleotides and can be followed easily by changes in absorption of ultraviolet light. Thus this method can be used to determine the structure oB tRNA's. A fingerprint of the N-oxidation product of tRNAFP,",', reveals that all adenosine residues are protected except the 3'-terminal adenosine and the three adenosine residues in or adjacent to the anticodon. On this basis a conformation of tRNA';,h,e,, is proposed. Similar tertiary structures can be constructed for the other tRNA's. In order to connect tertiary structure of a tRNA and recognition by its aminoacylating enzyme, the rate of aminoacylation, as a function of temperature, was measured. Neither changes in the anticodon nor specific changes at