✦ LIBER ✦

Structure and growth of ultrasmall protein microcrystals by synchrotron radiation: I. µGISAXS and µdiffraction of P450scc

✍ Scribed by Claudio Nicolini; Eugenia Pechkova

Publisher: John Wiley and Sons
Year: 2006
Tongue: English
Weight: 313 KB
Volume: 97
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.20537

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✦ Synopsis

Abstract

Ultrasmall P450scc cytochrome microcrystals are grown by classical hanging vapor diffusion and by its modification using homologous protein thin‐film template displaying a long‐range order. The nucleation and growth mechanisms of P450scc microcrystals are studied at the thin cytochrome film surface by a new microbeam grazing incidence small angle X‐ray scattering (µGISAXS) technique developed at the microfocus beamline of the European Synchrotron Radiation Facility (ESRF) in Grenoble, France. P450scc cytochrome crystals of about 5 µm are also investigated by synchrotron radiation diffraction in order to attempt a preliminary analysis of the atomic structure of this unique protein system yet unsolved. J. Cell. Biochem. 97: 544–552, 2006. © 2005 Wiley‐Liss, Inc.

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