Structure and expression of mos sequences in spontaneous and moloney murine sarcoma virus-induced yolk sac carcinomas in rats
✍ Scribed by B. Decock; H. Sobis; L. Van Hove; M. Vandeputte; A. Billiau
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- French
- Weight
- 990 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Facilitation of yolk-sac carcinoma (YSCa) development in fetectomized rats by the Moloney murine sarcoma virus/murine leukemia virus (Mo-MSV/MLV) complex was found to be closely associated with the presence of Mo-MSV sequences in the genomes of the YSCa cells. The virus-induced YSCas consisted of cells of mono-or oligoclonal origin which always contained in their genomes at least I randomly integrated Mo-MSV provirus. In YSCas which developed in the absence of virus, no rearrangement or amplification of c-mos could be detected. In addition, blot hybridization analysis of cellular RNA failed to detect mos-related RNA in cell lines derived from Mo-MSV-induced as well as from non-virally induced YSCas. The methylation level of c-mos D N A was low in all YSCa cell lines. In contrast, v-mos D N A in cell lines derived from Mo-MSV-induced YSCas was heavily methylated. 'To whom reprint requests should be sent.