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Structure and expression of an amylopullulanase gene from Bacillus stearothermophilus TS-23

✍ Scribed by Jen-Tao Chen; Ming-Chu Chen; Li-Lin Chen; Wen-Shen Chu


Book ID
111717826
Publisher
Portland Press
Year
2001
Tongue
English
Weight
439 KB
Volume
33
Category
Article
ISSN
0885-4513

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Cloning and expression of a thermophilic
✍ Tsukagoshi, N. ;Ihara, H. ;Yamagata, H. ;Udaka, S. πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 584 KB

A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. In attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 Kb HindIII