Structure and expression of a plastid-encodedgroELhomologous heat-shock gene in a thermophilic unicellular red alga

✍ Scribed by Udo Maid; Rolf Steinmüller; Klaus Zetsche

• Book ID: 104735398
• Publisher: Springer-Verlag
• Year: 1992
• Tongue: English
• Weight: 612 KB
• Volume: 21
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00351663

✦ Synopsis

A gene homologous to the E. coli groEL locus was identified on the plastid genome of the unicellular red alga Cyanidium caldarium strain 14-1-1 (synonym: Galdieria sulphuraria). The complete nucleotide sequence was determined and compared to bacterial-and nuclearencoded counterparts of higher plants. At the amino-acid level the C. caldarium gene shows 70% homology to the corresponding gene of the cyanobacterium Synechococcus and 52% homology to nuclear-encoded counterparts of higher plants, respectively. Northern and Western blot experiments were used to investigate the dependence of the transcript-and protein-level on culture temperature and heat shock.