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Structure and DNA-binding properties of the cytolysin regulator CylR2 from Enterococcus faecalis

✍ Scribed by Rumpel, Sigrun (author);Razeto, Adelia (author);Pillar, Chris M. (author);Vijayan, Vinesh (author);Taylor, Austin (author);Giller, Karin (author);Gilmore, Michael S. (author);Becker, Stefan (author);Zweckstetter, Markus (author)

Book ID: 110030759
Publisher: Nature Publishing Group
Year: 2004
Tongue: English
Weight: 594 KB
Volume: 23
Category: Article
ISSN: 0261-4189
DOI: 10.1038/sj.emboj.7600367

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## Abstract The bldB gene from __Streptomyces lividans__ was cloned, and its product was overexpressed in __Escherichia coli__ using a T7 expression system. Gel mobility shift assays showed that the BldB protein was functionally expressed in the __E. coli__ system and may negatively regulate its ow