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Structural transitions of concanavalin A adsorbed onto bare mica plates: surface force measurements

โœ Scribed by J. -P. Gallinet; B. Gauthier-Manuel


Book ID
104655315
Publisher
Springer
Year
1993
Tongue
English
Weight
440 KB
Volume
22
Category
Article
ISSN
1432-1017

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โœฆ Synopsis


The structure of the/~-sheet rich molecule, con-canavalinA (Con A), shows complicated behaviour when mechanical stress is imposed on adsorbed Con A layers. In the pH range 3.9 to 7.4, the structural changes are dependent on the pH and ionic strength of the solution at the liquid/solid interface. Several molecular shapes are observed ranging from a highly unfolded state characterized by a 1.25 nm thickness of each expelled layer at pH 3.9 to other states with various layer thicknesses at pH 6-7.4 and at a low ionic strength (< 10-4).

In the pH range 6 to 7.4, different molecular species appear to be present.


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