Structural stability and unfolding transition of β-glucosidases: a comparative investigation on isozymes from a thermo-tolerant yeast

Purification and characterization of two

Purification and characterization of two β-glucosidases from a thermo-tolerant yeast Pichia etchellsii

✍ Anu Wallecha; Saroj Mishra 📂 Article 📅 2003 🏛 Elsevier Science 🌐 English ⚖ 265 KB

The thermo-tolerant yeast Pichia etchellsii produced two cell-wall-bound inducible beta-glucosidases, BGLI (molecular mass 186 kDa) and BGLII (molecular mass 340 kDa), which were purified by a simple, three-step method, comprising ammonium sulfate precipitation, ion-exchange and hydroxyapatite chrom