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Structural requirements for allosteric activators of rat liver microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase

โœ Scribed by Joseph Roitelman; Ishaiahu Shechter

Book ID
115757964
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
343 KB
Volume
125
Category
Article
ISSN
0006-291X

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โœ Zafarul H. Beg; John A. Stonik; H.Bryan Brewer Jr. ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 312 KB

A simple, efficient, freeze-thaw procedure for the solubilization of liver 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase has been developed. Microsomes of chicken or rat liver were prepared by homogenization in buffer containing 100 mM sucrose, 50 mM KCI, 40 mM KH,PO,, 30 mM EDTA. and 2