✦ LIBER ✦

Structural properties of 2/2 hemoglobins: The group III protein from Helicobacter hepaticus

✍ Scribed by Henry J. Nothnagel; Benjamin Y. Winer; David A. Vuletich; Matthew P. Pond; Juliette T. J. Lecomte

Publisher: John Wiley and Sons
Year: 2011
Tongue: English
Weight: 766 KB
Volume: 63
Category: Article
ISSN: 1521-6543
DOI: 10.1002/iub.430

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The ε‐proteobacterium Helicobacter hepaticus (Hh) contains a gene coding for a hemoglobin (Hb). The protein belongs to the 2/2 Hb lineage and is representative of group III, a set of Hbs about which little is known. An expression and purification procedure was developed for Hh Hb. Electronic absorption and nuclear magnetic resonance (NMR) spectra were used to characterize ligation states of the ferric and ferrous protein. The p__K__~a~ of the acid/alkaline transition of ferric Hh Hb was 7.3, an unusually low value. NMR analysis of the cyanomet complex showed the orientation of the heme group to be reversed when compared with most group I and group II 2/2 Hbs. Ferrous Hh Hb formed a stable cyanide complex that yielded NMR spectra similar to those of the carbonmonoxy complex. All forms of Hh Hb were self‐associated at NMR concentrations. Comparison was made to the related Campylobacter jejuni 2/2 Hb (Ctb), and the amino acid conservation pattern of group III was reinspected to help in the generalization of structure–function relationships. © 2011 IUBMB IUBMB Life, 63(3): 197–205, 2011

📜 SIMILAR VOLUMES

Influence of Chelating Groups on the Lum

Influence of Chelating Groups on the Luminescence Properties of Europium(III) and Terbium(III) chelates in the 2,2′-bipyridine series

✍ Veli-Matti Mukkala; Marek Kwiatkowski; Jouko Kankare; Harri Takalo 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 German ⚖ 445 KB

## Abstract Eight different 2,2′‐bipyridine derivatives, __i.e__. 2, 5, 8, 10, 12, 13, 15, and 19 (__Schemes 1__ and __2__), were prepared to study the influence of the chelating groups on the luminescence properties of their Eu^III^ and Tb^III^ chelates. According to our luminescence results, 2,2′

ChemInform Abstract: The Utility of 2,2′

ChemInform Abstract: The Utility of 2,2′-Bipyrimidine in Lanthanide Chemistry: From Materials Synthesis to Structural and Physical Properties.

✍ Gael Zucchi 📂 Article 📅 2011 🏛 John Wiley and Sons ⚖ 18 KB 👁 2 views

Comparative modeling of the three-dimens

Comparative modeling of the three-dimensional structure of the calmodulin-related TCH2 protein from arabidopsis

✍ Amir R. Khan; Keith A. Johnson; Janet Braam; Michael N. G. James 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 292 KB 👁 2 views

Plants adapt to various stresses by developmental alterations that render them less easily damaged. Expression of the TCH2 gene of Arabidopsis is strongly induced by stimuli such as touch and wind. The gene product, TCH2, belongs to the calmodulin (CaM) family of proteins and contains four highly co

ChemInform Abstract: The Synthesis of [M

ChemInform Abstract: The Synthesis of [Me4N]2 [a2-B20H16(NH3)2] (II) and trans-B20H16(NH3)2 (III) from [Me3NH][cis-B20H17NH3] and Their Structural Characterization.

✍ Fangbiao Li; Kenneth Shelly; Carolyn B. Knobler; M. Frederick Hawthorne 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 37 KB 👁 1 views

Tripodal Trimanganese(III) Complexes of

Tripodal Trimanganese(III) Complexes of New Unsymmetrical Pentadentate Ligands Derived from 2-(Salicylideneamino)phenol: Syntheses, Crystal Structures and Properties

✍ Masahiro Muto; Naoya Hatae; Yumi Tamekuni; Yasunori Yamada; Masayuki Koikawa; Ta 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 234 KB 👁 1 views

Preparation, Structure, and Properties o

Preparation, Structure, and Properties of V2GeO4F2—Chains of VO4F2 Octahedra in the First V(III) Metallate Fluoride

✍ S.N. Achary; A.K. Tyagi; J. Köhler 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 331 KB

Powder samples of the new oxide fluoride V 2 GeO 4 F 2 have been obtained by the reaction of appropriate amounts V 2 O 3 , VF 3 , and GeO 2 at 7001C for 18 h in an argon-filled sealed platinum tube. V 2 GeO 4 F 2 crystallizes in the space group Pnma with a=9.336(1), b=8.898 (1), and c=4.912 (1) A ˚.