𝔖 Bobbio Scriptorium
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Structural organization of the genome of the zygomyceteAbsidia glauca: evidence for high repetitive DNA content

✍ Scribed by Johannes Wöstemeyer; Anke Burmester

Book ID
104756715
Publisher
Springer-Verlag
Year
1986
Tongue
English
Weight
409 KB
Volume
10
Category
Article
ISSN
0172-8083

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✦ Synopsis

Total cell DNA of Absidia glauca has a GC-content of 44.6% +/- 0.5% as determined from optical melting profiles which is in good accordance with values from equilibrium centrifugation in bisbenzimide containing CsCl gradients (46.2% +/- 1.1%), whereas mitochondrial DNA has a GC-content of only 30%. The genome size of Absidia glauca is approximately 36,000 kb, 8.6 times that of Escherichia coli. Three kinetically different fractions could be identified in reassociation experiments: a foldback-DNA fraction, comprising approximately 10% of the total DNA, repetitive DNA (25%) and single copy DNA (65%). This relatively high amount of repetitive DNA could partly be ascribed to ribosomal DNA (13%) and a new interspersed repetitive element ("rAg1") which has been cloned in pBR325.

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