Structural organisation of the extrinsic polypeptides from photosystem II
β Scribed by A. Seidler; A.W. Rutherford
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 58 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0162-0134
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Electron paramagnetic resonance (EPR) spectroscopy and 02 evolution assays were performed on photosystem II (PSII) membranes which had been treated with 1 M CaC12 to release the 17, 23 and 33 kilodalton (kDa) extrinsic polypeptides. Manganese was not released from PSII membranes by this treatment as
Selective solubilization of Photosystem II membranes with the non-ionic detergent octyl thioglucopyranoside has allowed the isolation of a PS II system which has been depleted of the 22 and 10 kDa polypeptides but retains all three extrinsic proteins (33, 23 and 17 kDa). The PS II membranes which ha