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Structural heterogeneity of chromatin preparations at the level of DNA topology

✍ Scribed by V. D. Paponov; G. A. Sigora; S. P. Rad'ko


Publisher
Springer US
Year
1987
Tongue
English
Weight
383 KB
Volume
104
Category
Article
ISSN
0007-4888

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Hydrodynamic shearing of chromatin in the presence of Mg(2+) ions produces two discrete types of particles: (1) molecules of completely free DNA which comprise 20-23% of the total DNA and (2) histone-covered DNA molecules which contain all five histone fractions. The average length of free DNA molec