Structural Elucidation of Clenbuterol- and Mabuterol-like Compounds in Complex Matrices Using Silylated andn-Butylboronate Derivatives by Gas Chromatography/Electron Impact and Chemical Ionization Mass Spectrometry
✍ Scribed by Saltron, Frédéric; Berthoz, Yann; Rues, Robert; Auguin, Nancy; Belhade, Loïck
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 729 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1076-5174
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✦ Synopsis
The mass spectra as their trimethylsilyl ethers and boronated derivatives under electron impact (Er) and methane chemical ionization conditions of seven unauthorized flz-agonists found in cattle feed are presented. Structural elucidation was achieved through comparison with the standards clenbuterol, mabuterol and mapenterol. The structures of brombuterol, bromoclenbuterol and bromomapenterol were deduced from their isotopic patterns. Examination of fragments of boronated mabuterol-like compounds under EI conditions involved the differentiation of two isobaric compounds, mahexerol and Imethylmapenterol. The amino substituent of isopropylclenbuterol and isopropylbromoclenbuterol was obtained from clenbuterol, as these molecules specially fragment with a neutral dissociation of the side-chain, confirmed by tandem mass spectrometry on a triple-quadrupole instrument. The branching structure of isopropylclenbuterol was investigated by means of 'H NMR experiments.