𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Structural Characterization of Site-Specific N-Glycosylation of Recombinant Human Factor VIII by Reversed-Phase High-Performance Liquid Chromatography−Electrospray Ionization Mass Spectrometry

✍ Scribed by Medzihradszky, Katalin F.; Besman, Marc J.; Burlingame, A. L.


Book ID
125850692
Publisher
American Chemical Society
Year
1997
Tongue
English
Weight
558 KB
Volume
69
Category
Article
ISSN
0003-2700

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Analysis of Recombinant Human ADP-Ribosy
✍ Scott J. Berger; Alejandro C. Claude; Paul Melançon 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 271 KB

Two complementary approaches utilizing reverse-phase high-performance liquid chromatography and liquid chromatography/mass spectrometry were developed to analyze recombinantly produced Group I and Group II human ADP-ribosylation factors (ARFs). We observe that the NH2 termini of Group II ARFs (ARF4

Reverse-phase capillary high performance
✍ Katalin F. Medzihradszky; Marc J. Besman; A. L. Burlingame 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 226 KB 👁 1 views

The B-domain of recombinant human Factor VIII comprises 909 amino acids and is extensively N-and Oglycosylated, in that at least 20 different sites are occupied by numerous carbohydrate structures. This domain was incubated with trypsin and subjected to liquid chromatography electrospray ionization