Two complementary approaches utilizing reverse-phase high-performance liquid chromatography and liquid chromatography/mass spectrometry were developed to analyze recombinantly produced Group I and Group II human ADP-ribosylation factors (ARFs). We observe that the NH2 termini of Group II ARFs (ARF4
✦ LIBER ✦
Structural Characterization of Site-Specific N-Glycosylation of Recombinant Human Factor VIII by Reversed-Phase High-Performance Liquid Chromatography−Electrospray Ionization Mass Spectrometry
✍ Scribed by Medzihradszky, Katalin F.; Besman, Marc J.; Burlingame, A. L.
- Book ID
- 125850692
- Publisher
- American Chemical Society
- Year
- 1997
- Tongue
- English
- Weight
- 558 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0003-2700
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The B-domain of recombinant human Factor VIII comprises 909 amino acids and is extensively N-and Oglycosylated, in that at least 20 different sites are occupied by numerous carbohydrate structures. This domain was incubated with trypsin and subjected to liquid chromatography electrospray ionization