An arabinoxylan-rhamnogalacturonan complex, comprised of galacturonic acid, rhamnose, arabinose, xylose, and galactose in the ratios 75.9:4.6:5.2:3.5:5.4 and lesser amounts of other constituents, was dissociated from the water-insoluble matrix of cell walls of Zea mays by xylanase and glucuronoxylan
Structural characterization of feruloyl oligosaccharides from spinach-leaf cell walls
โ Scribed by Tadashi Ishii; Tetsuya Tobita
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 754 KB
- Volume
- 248
- Category
- Article
- ISSN
- 0008-6215
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โฆ Synopsis
Hydrolysis of spinach-leaf cell walls with Driselase (a fungal enzyme preparation) released two arabino-oligosaccharides and one galactobiose, each carrying a ferulic acid moiety. The oligosaccharides were characterized by NMR spectroscopy, methylation analysis, and FABMS. They were O-(2-O-trans-feruloyl-alpha-L-arabinofuranosyl)-(1-->5)-L-arabinof uranose, O-(6-O-trans-feruloyl-beta-D-galactopyranosyl)-(1-->4)-D-galactopy ranose, and O-alpha-L-arabinofuranosyl-(1-->3)-O-(2-O-trans-feruloyl-alpha-L-a rabino- furanosyl)-(1-->5)-L-arabinofuranose.
๐ SIMILAR VOLUMES
Rhamnogalacturonan I (RG-I), a pectic polysaccharide isolated from the walls of suspension-cultured sycamore cells, was shown by glycosyl-residue composition analysis to contain D-glucosyluronic acid (GlcpA) residues (1 mol%) and 4-O-methyl-D-glucosyluronic acid (4-O-Me-GlcpA) residues (0.5 mol%). T