Structural characterization of a rice actin gene

✍ Scribed by David McElroy; Madge Rothenberg; Ray Wu

• Book ID: 104614822
• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 1022 KB
• Volume: 14
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00018557

✦ Synopsis

We have isolated and sequenced a full-length cDNA clone containing information for the rice actin gene RAcl. Transcript terminus mapping and sequence alignment between the RAcl cDNA clone and a previously isolated RAcl genomic clone were used to determine the structure of the RAcl gene. This allowed us to make the first complete structural characterization of a plant actin gene. The analysis revealed the presence of a 5'-noncoding exon, separated by an intron, from the first translated exon of the RAc 1 gene. This is one of the few reported cases of a plant gene containing such a 5'-noncoding exon. Sequence comparison between the previously isolated plant actin genes suggests that such an exon may be a common feature of plant actin gene structure. The present study also confirms that the rice actin gene family is composed of at least eight unique members.