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Structural basis of substrate specificity in malate dehydrogenases: crystal structure of a ternary complex of porcine cytoplasmic malate dehydrogenase, α-Ketomalonate and TetrahydoNAD

✍ Scribed by A.D.M Chapman; A Cortés; T.R Dafforn; A.R Clarke; R.L Brady


Book ID
115628971
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
897 KB
Volume
285
Category
Article
ISSN
0022-2836

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The crystal structure of a thermophilic alcohol dehydrogenase (TBAD) from Thermoanaerobacter brockii has been determined in a binary complex with sec-butanol as substrate to a resolution of 3.0 A ˚. Van der Waals interactions of the carbon C1 atom of sec-butanol with atoms in His59, Ala85, Trp110, A