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Structural basis for the bifunctionality of fructose-1,6-bisphosphate aldolase/phosphatase

✍ Scribed by Fushinobu, Shinya; Nishimasu, Hiroshi; Hattori, Daiki; Song, Hyun-Jin; Wakagi, Takayoshi

Book ID
121416727
Publisher
Nature Publishing Group
Year
2011
Tongue
English
Weight
854 KB
Volume
478
Category
Article
ISSN
0028-0836

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A one-step procedure for the isolation o
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Adsorption to blue dextran-Sepharose and successive elution with AMP and fructose 1,6-bisphosphate provides a one-step procedure for the simultaneous purification of fructose 1,6-bisphosphatase and fructose 1,Cbisphosphate aldolase from rabbit liver homogenates. The procedure can be completed in a s