Structural and molecular properties of a water-soluble arabinoxylan-protein complex isolated from rye bran
✍ Scribed by Anna Ebringerová; Zdenka Hromádková; Gisela Berth
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 864 KB
- Volume
- 264
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
The main structural features of the water-soluble arabinoxylan-protein complex isolated from rye bran were evaluated by means of methylation analysis and 13C NMR spectroscopy. A highly branched arabinoxylan with Araf residues in mono-and di-substitution positions and side chains terminated with Xylp groups represents the main component associated with a serine-and glycine-rich cell-wall protein. More than 30% of the arabinose is linked in various positions probably as oligosaccharide side-chains and/or branched (1 + 5)-arabinan. The molecular parameters were estimated by HPGPC as well as by gel chromatography combined with viscometry and light-scattering.
The high molecular weight fraction close to the exclusion volume (a, > 106), which is shifted after treatment with Pronase to the region lo4 < &?W < 106, is considered to consist of fragments of the native cell-wall matrix with arabinoxylan chains probably linked to a protein core.