Structural and functional domains on actin

✍ Scribed by Brett D. Hambly; Julian A. Barden; Masao Miki; Cristobal G. Dos Remedios

• Book ID: 102759280
• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 612 KB
• Volume: 4
• Category: Article
• ISSN: 0265-9247
• DOI: 10.1002/bies.950040308

✦ Synopsis

Actin plays several essential roles in cdluiarprocesses and is a vital component in the contractile apparatus. To accomplish its many cellular tasks, actin must interact with a wide range of other proteins in addition to self-assembling into $laments. Characterization of these functional domains and localized binding regions on the actin monomer is therefore an imporlanl undertaking. Strategies for elucidating the many interaction sites include X-ray dij?raction, NMR and jluorescence spectroscopy, chemical mod-$cation, chemical cross-linking, protein cleavage, and the study of sequence homologies between the many isotypes 05

actin. Based on these varied data, we discuss the possible spatial relationships between the interaction sites.