✦ LIBER ✦

Structural analysis of sulfonated monoazo dyestuff intermediates by electrospray tandem mass spectrometry and matrix-assisted laser desorption/ionization post-source decay mass spectrometry

✍ Scribed by Anthony. G. Sullivan; Robert Garner; Simon. J. Gaskell

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 212 KB
Volume: 12
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19980915)12:17<1207::aid-rcm310>3.0.co;2-x

No coin nor oath required. For personal study only.

✦ Synopsis

Sulfonated azo molecules containing hydroxy groups ortho-or para-to the azo linkage exhibit azo/ hydrazone tautomerism, which affects their coloristic properties and also the fragmentation pathways observed in tandem mass spectrometry. Electrospray tandem MS and matrix-assisted laser desorption/ ionization post-source decay are used in this work to study the fragmentation mechanisms of such molecules. The two techniques show closely matching product ion distributions, the predominant fragmentation pathways depending on the extent of tautomerism in each molecule. Evidence of tautomerism is afforded by comparing the product ions of the hydroxyazo molecules with their methoxyazo analogues where tautomerism is not possible. Keto-tautomers show cleavage of the single N-N bond whereas the azo forms dissociate via the azo C-N bonds, followed in both cases by neutral losses of SO 2 . In addition, the simple introduction of a nitro group on a naphthyl group in an azo dye is shown to have a marked influence on its observed fragmentation.

📜 SIMILAR VOLUMES

Structure analysis of trivalent glycoclu

Structure analysis of trivalent glycoclusters by post-source decay matrix-assisted laser desorption/ionization mass spectrometry

✍ Havlíček, Vladimír; Kieburg, Christoffer; Novák, Petr; Bezouška, Karel; Lindhors 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 270 KB 👁 2 views

Post-source decay (PSD) matrix-assisted laser desorption/ionization time-of-Ñight mass spectra were found to be useful for the structural elucidation of a series of tris [ 2-(glycosylthiourylene)ethyl ] amines. The reported fragmentation behaviours of [ M + H ] ', [ M + Na ] ' and [ M Ô H ]ions di †

Structural Analysis of Derivatized Oligo

Structural Analysis of Derivatized Oligosaccharides Using Post-source Decay Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

✍ Lemoine, J.; Chirat, F.; Domon, B. 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 437 KB 👁 2 views

Structural information on oligosaccharides was obtained by post-source decay matrix-assisted laser desorp tion/ionization mass spectrometry. A systematic study of derivatized oligosaccharides showed that products relatively aminated with benzylamine allow the formation of [ M + HI + molecular specie

The Analysis of Polysulfonated Azo Dyest

The Analysis of Polysulfonated Azo Dyestuffs by Matrix-assisted Laser Desorption/Ionization and Electrospray Mass Spectrometry

✍ Anthony G. Sullivan; Simon J. Gaskell 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 196 KB 👁 1 views

Electrospray ionization and matrix-assisted laser desorption/ionization have been applied to the analysis of polysulfonated azo dyestuff mixtures. Three homologous orange dyestuffs were synthesized using a harsh, two-stage process involving a condensation followed by a reduction and, producing compl

Fragmentation and sequencing of cyclic p

Fragmentation and sequencing of cyclic peptides by matrix-assisted laser desorption/ionization post-source decay mass spectrometry

✍ Birgit Schilling; Wei Wang; John S. McMurray; Katalin F. Medzihradszky 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 104 KB 👁 2 views

A series of synthetic cyclic decapeptides and other smaller cyclic peptides were analyzed using matrixassisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The investigated compounds were cyclized in a head-to-tail manner and contained non-proteinaceous amino acids, such

Protein identification based on matrix a

Protein identification based on matrix assisted laser desorption/ionization-post source decay-mass spectrometry

✍ Kris Gevaert; Hans Demol; Lennart Martens; Bart Hoorelbeke; Magda Puype; Marc Go 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 104 KB 👁 1 views

Accurate peptide sequencing by post-sour

Accurate peptide sequencing by post-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Wenjun Mo; Toshifumi Takao; Yasutsugu Shimonishi 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 153 KB 👁 2 views

Partial 18 O-labeling of peptides has been applied to post-source decay experiments in a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer. The ions which originate from the carboxyl terminus of a peptide partially retain 18 O atoms which have readily been incorporated int