Structural analysis of pectic polysaccharides by base-catalysed β-elimination in the presence of sodium borodeuteride

✍ Scribed by Hiroaki Kiyohara; Haruki Yamada

• Book ID: 102995572
• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 868 KB
• Volume: 187
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(89)80059-x

✦ Synopsis

A methyl-esterified rhamnogalacturonan with neutral carbohydrate side-1988) chains (PG-l), derived from an acidic pectic polysaccharide (AR-411~) from a hotwater extract of Angelica acutiloba Kitagawa, was subjected to base-catalysed pelimination in the presence of sodium borodeuteride. The neutral carbohydrate chains, thereby released from position 4 of GalA residues and isolated by anionexchange chromatography, were shown by gel-filtration chromatography and methylation analysis to consist of a high-molecular-weight galactan with some arabinosyl side-chains and material of lower molecular weight which consisted mainly of branched arabinosyl chains. Partial hydrolysis of PG-1 with acid gave a rhamnogalacturonan (MPG-l) possessing short oligosaccharide chains. P-Eliminative degradation of MPG-l released neutral oligosaccharide chains terminated by galactitol-l-d and rhamnitol-Z-d moieties. G.l.c.-m.s.